Clinical Chemistry > LECTURE SLIDES/NOTES > Liver Function Test detailed notes (All)
Liver Function Test detailed notes
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USED TO Detect presence of liver disease Distinguish among different types of liver ds Gauge the extent of known liver damage Follow the response of treatment R ... arely suggest a specific diagnosis functions Serum bilirubin Urine bilirubin Blood ammonia Serum enzymes : AST, ALT, GGT, 5’Nucleotidase,ALP of liver Serum Albumin Serum Globulins PT ,INR A break down product of porphyrin ring of heme – containing proteins , found in blood in 2 fractions – conj/unconj Conjugated : water soluble , so excreted by kidneys Unconjugated : insoluble in water , bound to albumin in blood About 300 mg of bilirubin is formed per day Normal total serum bilirubin: 0.3 – 1.3 mg/dl Direct/conjugated bilirubin: 0.1 – 0.4 mg/dl Indirect/unconjugated bilirubin: 0.2 – 0.9mg/dl Measured by Van Den Bergh method Bilirubin reacts with diazo reagent to produce coloured azo pigment . At pH 5 – pigment purple . [Show More]
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