*NURSING > Study Notes > Chamberlain College of Nursing BIOS 242 biology midterm . (All)

Chamberlain College of Nursing BIOS 242 biology midterm .

Document Content and Description Below

Know the experimental design Louis Pasteur developed to disprove spontaneous generation. Pasteur filled flasks with broth and fashioned their openings into long, swan-neckshaped tubes. The broth in ... the flasks was still open to the air but curved so that gravity would deposit any contaminants in the neck of the flask. He boiled the flasks to sterilize the broth and incubated them to encourage the growth of microbes. As long as the neck of the flask was intact, no microbes grew. If the swan-necked flask was broken off so that dust could fall into the container, microbes grew, and the broth became cloudy.  List Koch’s postulates and apply them and know the contributions by Antoni Von Leeuwenhoek. Koch’s postulates:  Find evidence of a particular microbe in every case of a disease.  Isolate that microbe from an infected subject and cultivate it in pure culture in the laboratory; perform full microscopic and biological characterization.  Inoculate a susceptible healthy subject with the laboratory isolate and observe the same resultant disease.  Reisolate the same agent from this subject. Antoni Von Leeuwenhoek:  Made a crude microscope to examine threads in fabrics  Made drawings of what he called “animalcules” in rainwater and scraped from his teeth  Define: parasites, inoculation, generation time, prions, sterilization, disinfection, endotoxin, fomite. Parasites: An organism that lives on or within another organism (the host), from which it obtains nutrients and enjoys protection. The parasite produces some degree of harm in the host. Inoculation: Introduction of microbes into media for culture Generation time: Time required for a complete fission cycle—from parent cell to two new daughter cells. Also called doubling time. Prions: A concocted word to denote “proteinaceous infectious agent”; a cytopathic protein associated with the slow-virus spongiform encephalopathies of humans and animals. Sterilization: Any process that completely removes or destroys all viable microorganisms, including viruses, from an object or habitat. Material so treated is sterile [Show More]

Last updated: 2 years ago

Preview 1 out of 13 pages

Buy Now

Instant download

We Accept: