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WGU BioChem OA Review with Complete and Verified Solutions

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WGU BioChem OA Review with Complete and Verified Solutions Males and Females are equally affected ✔✔Autosomal The process of copying DNA in the lab. ✔✔Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS) ...

WGU BioChem OA Review with Complete and Verified Solutions Males and Females are equally affected ✔✔Autosomal The process of copying DNA in the lab. ✔✔Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. ✔✔What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating ✔✔What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) ✔✔What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick to the DNA that you want to copy and ADD DNA Polymerase ✔✔What is Annealing? The Polymerase Chain Reaction is heated and DNA Polymerase adds nucleotides to build a new DNA strand. ✔✔What is elongating? Ligase ✔✔What is NOT involved in PCR? It is used to repair damage to bases caused by harmful molecules by removing the base that is damaged and replacing it. #1 GLYCOSYLASES see the damaged DNA. #2 DNA Polymerase puts the right one back #3 DNA Ligase seals it. ✔✔What is base excision repair (BER)? DNA Glycolysase sees the damage and removes it, DNA polymerase puts the tight base back, DNA ligase seals it back up. ✔✔What are the 3 steps in base excision repair? 1 ✔✔How many nucleotides does base excision repair remove? Mismatch Repair (MMR). It is the only one to occur during REPLICATION - DURING THE PROOFREADING ✔✔What is it called when a large section of the nucleotide is removed from the DNA so that DNA polymerase can try again? (This corrects DNA damage) Mismatch Repair (MMR) repairs errors such as G with T instead of G with C. DNA Polymerase fixes it. This happens during replication and MMR removes a large section of the nucleotide from the DNA and DNA Polymerase tries again. ✔✔What repairs a base mismatch? When DNA polymerase takes an individual nucleotide and matches them to the parental sequences to ensure a correct pair. (it must bind with RNA primer to work). ie: DNA Polymerase binds to DNA to make RNA ✔✔What is DNA Transcription?

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