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MICROBIOLOGY LAB 1 ,9

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TK01 Keeping a Lab Notebook Microbiology Lab1 Course Objectives: Cultivation of samples (growth conditions) - equipment used Identification of samples (biochemical assays) - tests available Evaluat ... ion of samples (microscopy) - visualizations and recognitions key concepts Basic Equipment: 1. Cleaning: autoclave 125 ° C, steamed, heat and pressure sterilization, chamber with thick door, not dry air but steamed - more efficient, make sure depressurized before opening Time restrictions: no autoclave then use steam (minutes) vs. hot air (hours) 2. Growing: fixed incubator = 37 ° C; shaker incubator = 37 ° C, for growth mediator, rotates and shakes in circular motions to aerate and help optimize bacterial growth 3. Visualizing: Microscopy, can't see with just the naked eye, must know parts of microscope 4. Storing: Refrigerate at 4 ° C, slows bacterial growth, prolongs life of sample at this temperature, when not finished with experimentation Lab Safety: 1. Never eat or drink in lab - contamination risks 2. Wear Personal Protective Equipment (PPE): Use gloves: latex, nitrile (hypoallergenic) purple,blue or green, cold thermal - liquid nitrogen, etc., heat thermal resistant gloves - extreme hot conditions Eyewear protection - goggles Lab coat - dependent on working with materials that may be spilt on clothes 3. NEVER leave lab while wearing PPE includes: Public Hallway, Restroom, Cafeteria Lab Notebook: First Name Initial, Last name initial, Experiment #, Title of Experiment Example: TK01 Keeping a Lab Notebook Objective: Developing an organized lab notebook for documenting experiments, data, procedures and records. [Show More]

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