Biology > Lab Experiment > BIOL 1020 Lab: Microscopy and Diffusion – Thinking About Scale (All)

BIOL 1020 Lab: Microscopy and Diffusion – Thinking About Scale

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Basic Techniques of Light Microscopy No questions to submit for this section; ensure that you work through the practice puzzles and ‘Test Your Understanding’ questions in the online content. Mea ... suring What You See: Viewing Magnification, Field of View, and Specimen Size 1. On the UD Virtual Compound Microscope, examine the onion root tip slide at 400X viewing magnification. Ensure that the image is centred. Assuming that the typical fields of view listed in the lab apply to the UD Virtual Compound Microscope, what is the approximate size of an onion root tip cell? Show your work and specify which dimension of the cell (length or width) you are using. The measurement I am using is length. The number of specimen is 11. The field view would equate to 0.35 mm because we are looking at the onion root tip at 400x magnification. So 0.35mm/11 specimen= 0.032mm. Therefore, the actual size of the onion root tip is 0.032mm. 2. Work through the Measurement activities m1 – m3 on the UD Virtual Microscope ‘Try This’ screen, and report your answers below: RESPONSE: m1 (height of letter e): 145 micrometers m2 (diameter of onion root tip cell in anaphase): 8 micrometers m3 (air bubble diameter) 2 micrometres 3. Return to the m2 activity. Use the ocular micrometer to measure the diameter of a non-dividing onion root tip cell nucleus and report your answer below. RESPONSE: 4 micrometres 4. Return to the m3 activity. Using the ocular micrometer, what is the approximate length of a bacterium, including the capsule? RESPONSE: 1 micrometer [Show More]

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