Biology > Lab Report > PCR Cloning and Expression of a GFP fusion protein in E. coli Hunter College, CUNYBIO 203Lab Report (All)
PCR Cloning and Expression of a GFP fusion protein in E. coli Hunter College, CUNYBIO 203Lab Report II=post
Document Content and Description Below
Lab Report II (Biol 203, Spring 2020) NAME (First, LAST): Sessions 9,10 PCR Cloning and Expression of a GFP fusion protein in E. coli PART I (No lab data required)- 60 points 1. Below is a map fo... r a plasmid called ‘pGFP’: The sequence (5’ to 3’) of pGFP containing the promoter region, the 5’ multiple cloning site (with a BamHI site, underlined), and the 5’ end of the GFP coding sequence is as follows: 1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGA GCGCAA CGCAATTAAT GTGAGTTAGC 121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGG (lac P) A(+1)A 181 TTGTGAGCGG ATAACAAT (lacO) TT CACAC AGGA (SD) A ACAGCTATG (lacZ#1) A CCATGATTAC GCCAAGCTTG 241 CATGCCTGCA GGTCGACTCT AGAGGATCCC CGGGTACCGG TAGAAAAAAT G (GFP#1) AGTAAAGGA 301 GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA TGTTAATGGG 361 CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA ACTTACCCTT [Show More]
Last updated: 2 years ago
Preview 1 out of 9 pages
Buy this document to get the full access instantly
Instant Download Access after purchase
Buy NowInstant download
We Accept:
Also available in bundle (1)
PCR Cloning and Expression of a GFP fusion protein in E. coli
PCR Cloning and Expression of a GFP fusion protein in E. coli
By Muchiri 4 years ago
$10
3
Reviews( 0 )
$7.00
Can't find what you want? Try our AI powered Search
Document information
Connected school, study & course
About the document
Uploaded On
Apr 22, 2021
Number of pages
9
Written in
Additional information
This document has been written for:
Uploaded
Apr 22, 2021
Downloads
0
Views
80
.png)
