Biology > Lab Report > Queens College, CUNY BIO 203Bio 203 lab report 2 II-REVISED BR EXPERTS-GRADED A (All)

Queens College, CUNY BIO 203Bio 203 lab report 2 II-REVISED BR EXPERTS-GRADED A

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PCR Cloning and Expression of a GFP fusion protein in E. coli PART I (No lab data required)- 60 points 1. Below is a map for a plasmid called ‘pGFP’: The sequence (5’ to 3’) of pGFP contai ... ning the promoter region, the 5’ multiple cloning site (with a BamHI site, underlined), and the 5’ end of the GFP coding sequence is as follows: 1 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 61 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 121 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA 181 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG 241 CATGCCTGCA GGTCGACTCT AGAGGATCCC CGGGTACCGG TAGAAAAAAT GAGTAAAGGA 301 GAAGAACTTT TCACTGGAGT TGTCCCAATT CTTGTTGAAT TAGATGGTGA TGTTAATGGG 361 CACAAATTTT CTGTCAGTGG AGAGGGTGAA GGTGATGCAA CATACGGAAA ACTTACCCTT 1The GFP open reading frame was cloned as a BamHI (5’ MCS)/ XhoI (3’MCS) fragment. In this plasmid, GFP is expressed as a fusion protein with LacZ. Clearly indicate on the above sequence the following elements: lac Promoter : lacP The start codon for LacZ: LacZ#1 [Show More]

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