BioChemistry > QUESTIONS & ANSWERS > Biochemistry C 785 Readiness Check 2022 Biochemistry C785 Readiness Check - A Grade (All)

Biochemistry C 785 Readiness Check 2022 Biochemistry C785 Readiness Check - A Grade

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The 2nd OA Readiness Check is designed to give you additional practice with new assessmentstyle questions to help prepare for success on the OA. Thus, please try to mimic examlike conditions by ... taking the test in one <2 hr sitting without any study aids apart from your whiteboard/scrap paper. Note: You can take the 2nd OA Readiness Check again if you're so inclined, but you can not edit your response as you may have done with the topic questions. You will be able to see the results afterwards. 1. What is the complementary sequence to 3’ ATG CGA ATA 5’? * 1/1 3’ TAT TCG CAT 5’ 3’ TAC GCT TAT 5’ 3’ GAT AGC ATA 5’ 5’ ATA AGC GTA 3’ Feedback The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (AT, GC, 5'3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’ 2. Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’? * 1/1 Tyr Val Tyr Ile Gln Ile Ile Asp Val Leu His Lys Feedback The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are noncomplementary and parallel. So we copy the coding strand , change T > U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' > 3') 3. Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’? * 0/1 Tyr Tyr Arg Ile Met Ala Ser Val Ile Ser His Gln Correct answer Ser Val Ile Feedback The correct answer is C because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile 4. Which of the following are the correct components for a PCR reaction? * 1/1 dNTPs, Primer, RNA Polymerase, template RNA dNTPs, Primer, DNA Polymerase, template DNA [Show More]

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