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CSD 06160 Microscope_Cell_LR. BioLab3 Microscope and the Cell Lab Report

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BioLab3 Microscope and the Cell Lab Report Name, date, course and section required for password: I. Purpose of the Microscope 1. Describe the correct way to handle a ... microscope. 2. Compare magnification, resolution, and contrast. II. Compound Microscope 1. Describe the location and function of the following parts of the microscope: a. ocular – b. condenser – c. stage – d. course adjustment knob 2. Describe how total magnification is calculated. III. Viewing Microscope Slides 1. Name the two types of slides used with the compound light microscope. 2. Describe in at least five steps, how to focus a microscope in order to view leaf cells on high magnification. 3. Compare the terms parfocal and parcentral. IV. Depth of Field 1. What was the purpose of viewing the slide with three colored threads? V. Microscopic Measurement 1. What is the diameter of the field of view under scanning power in micrometers (µm)? 2. A specimen fills 1/2 of the field of view under high power (400X). What is the specimen size in µm? VI. Cells 1. Describe the three parts of the modern cell theory. 2. Identify and describe the two major cell types. 3. What three parts do all cells have in common? VII. Prokaryotic Cells 1. What type of microscope should you use to view the shape of a bacterial colony? 2. Draw and label a typical bacterial cell, then provide functions for at least five of the labeled structures. Sign, date and prepare an image of your drawing and include it with this lab report. VIII. Eukaryotic Cells 1. Explain how to make a wet mount of cheek cells. What are two ways to increase contrast? Summary Questions 1. Explain how the microscope is used as an instrument of measurement. 2. Determine the total magnification of a microscope that has an eyepiece lens (10X) and an objective lens (10X). 3. Name three structures common to all cells. 4. Name three differences between prokaryotic and eukaryotic cells. 5. You are observing an onion epidermal cell under the microscope, but the image is faint. What could you do to improve contrast? 6. Compare the relative size of a bacteria cell, human cheek cell, and Elodea cell. 7. Describe three differences between plant and animal cells. Animal Cell Plant Cell 1. No chloroplast 1. chloroplast 2. No cell wall 2. Cell wall 3. No central vacuoles 3. Central vacuoles 8. Do all plant cells contain chloroplasts? [Show More]

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