Engineering > Research Paper > Worcester Polytechnic Institute - BME 2210BME2210_B17_HW5. (All)
Worcester Polytechnic Institute - BME 2210BME2210_B17_HW5.
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WPI Biomedical Engineering BME2210-B17 p. 1 HOMEWORK 5 Due: 11/27/2017 (Monday, 9pm) 1. The figure below shows the absorption spectra for an assay to determine the concentration of Ruthenium ions ... , [Ru3+], in a sample, by the addition of the dye per-6-amino-β-CyD/pnitrophenol. Colored curves indicate absorbance spectra from 0.5 – 5.0×10 -5 M or 5 – 50 µM [Ru3+]. 50 µM [Ru3+] 50 µM [Ru3+] The following table indicates the absorbance at different wavelengths and concentrations: Concentration [Ru3+] A(315nm) A(350nm) A(400nm) 10 µM 0.21 0.33 0.68 30 µM 0.41 0.33 0.36 50 µM 0.54 0.33 0.17 a. Using the table above, sketch three calibration curves at 400 nm, at 315 nm, and at 350 nm (Absorbance vs. [Ru3+]). https://www.coursehero.com/file/32968738/BME2210-B17-HW5docx/ This study resource was shared via CourseHero.comWPI Biomedical Engineering BME2210-B17 p. 2 b. What is the approximate sensitivity (Absorbance unit per M [Ru3+]) at each of these wavelengths, between 10 and 50 µM? [Show More]
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